Leading experts in custom designed quantitative real-time RT-PCR kits
- Primer Specificity: Highly specific primers are designed by avoiding significant cross homologies found by automatically interpreting BLAST search results.
- Primer Efficiency: Template secondary structures may hinder the primers from annealing and extension, and hence prevent the complete product formation by polymerases. The design avoids regions which may fold upon themselves at lower temperatures, thereby increasing the efficiency of the RT-PCR primers.
Each kit comes with the primer pair and standard for the gene of interest, as well as SYBR green master mix for 100 reactions (25 ul).
Below are some example of kits available. Kits can be custom designed for your gene of interest. Call or email us to place an order!
(248) 894-1474 / orders@dsbiotech.net
Oxidative Stress Markers
NADPH Oxidase 1 (NOX1)
Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Two transcript variants encoding different isoforms have been found for this gene.
NADPH Oxidase 2 (NOX2)
This gene is also known as CYBB cytochrome b-245 (CYBB). Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. (References: PMID: 21482679 PMID: 19041781)